Gene Targeting Strategy
The Nphs2 gene encodespodocin, a protein that is expressed at the insertion of the slit diaphragm in the renal glomerulus. Podocytes are specialized epithelial cells covering the basement membrane of the renal glomerulus, and they mediate glomerular filtration through the slit diaphragm. Podocin is thus crucial in the establishment of the glomerular filtration barrier. In this strain, Cre recombinase expression is under the control of Nphs2 promoter. When crossed with a strain containing a loxP site-flanked sequence of interest, Cre-mediated recombination results in deletion of the flanked sequence in Nphs2 expressing cells.
Rats hemizygous for Nphs2CreERT2 gene are viable and fertile without any physical abnormalities.
(B-CR-005) B-Nphs2(podocin)-CreERT2 rats