LAG-3 (Lymphocyte activation gene 3, CD223) is a lymphocyte activation gene and a member of the Ig family. It is mainly expressed in the activated T cells, NK cells, B cells and plasmacytoid DCs. LAG-3 is a negative regulator of immunity and binds to MHC class Ⅱ molecules to regulate the function of dendritic cells. The expression of LAG-3 is associated with the negative immunoregulatory function of specific T cells. Inhibition of LAG-3 function enhances the anti-tumor effect of specific CD8+ T cells, which is a potential tumor immunotherapy target.
Protein expression verification:
Fig 1. Spleen cells from both wild type (WT) C57BL/6 and the B-hLAG-3 heterozygous mice were analyzed by flow cytometry. Mouse LAG-3+ T cells were detected in both the WT and the B-hLAG-3 heterozygous mice, while human LAG-3+ T cells were only detected in the B-hLAG-3 homozygous mice.