Primate-specific viruses including HBV and HIV do not work well in preclinical efficacy evaluations in mice because of the host specificity, therefore impeding the drug development process. In addition, cellular immune therapy is becoming a major trend for tumor therapy, but the lack of immune system animal models has been a big obstacle for immunological therapeutic efficacy evaluation.
B-NDG mice is one of the most suitable tools for human-derived cells or tissue transplantation. Human immune reconstruction can be performed by transplanting human peripheral blood mononuclear cells (PBMCs) and human hematopoietic stem cells (hHSCs) into B-NDG mice. This powerful tool allows researchers to examine the human immune system and related primates' alien virus research and cellular immunotherapy preclinical evaluation.
Immune humanized B-NDG mice via hPBMCs transplantation
Fig 1. Human peripheral blood mononuclear cells (hPBMCs) were caudal vein injected (5x106 cells) into B-NDG mice for reconstitution experiments. The ratio of hCD45+ and mCD45+ was compared after 24 days. hPBMCs reconstituted well in 3 B-NDG mice and the major subsets of hPBMCs developed are T cells.