CD27 gene function:
CD27 (CD27 molecule) is a TNF receptor superfamily member. It is expressed on the surface of T and B cells, and is very important for producing and maintaining long-term T cell immunity. CD27 and its ligand CD70 are combined to provide a co-stimulation signal for T and B cell multiplication and for B cells to produce immunoglobulins. Experiments have demonstrated that a CD27 active type antibody can effectively activate the mouse tumor immune response. Treating mice with a CD27 active type antibody can effectively enhance the antitumor immunity effect for lymph cancer and B16 melanoma, providing putative targets for tumor immunological therapy.
Targeting strategy ：
mRNA expression verification:
Fig 1. Molecular identification of B-hCD27 humanized mice. mCD27mRNA expression was detected in spleen cells of B-hCD27 heterozygous mice.
Protein expression verification:
Fig 2. B-hCD27 humanized mice spleen cell activation and flow cytometry.
mCD27+ cells were detected in C57BL/6 mice and B-hCD27 heterozygous mice. hCD27+ cells were detected in B-hCD27 heterozygous mice.
Fig 3. B-hCD27 mice were used for hCD27 antibody efficacy validation. Mouse colon cancer MC38 cells were subcutaneously transplanted into B-hCD27 heterozygous mice, and animals were grouped into control to treatment groups (n=5) when the tumor size is approximately 100 mm3. Anti-hCD27 antibody shows substantially inhibitory effects. The average±SEM of tumor sizes are shown in the figure. B-hCD27 mice are effective tools for hCD27 antibody efficacy validation.