TNFRSF18 (TNF Receptor Superfamily Member 18) is also known as Glucocorticoid-induced TNFR-related protein (GITR), which is expressed on many immune cells including T cells. As a co-stimulatory signal of T cells, TNFRSF18 is up-regulated upon the activation of T cells, and in turn promotes T cell proliferation. CD25+/CD4+ regulatory T cell is known to mediate immune tolerance, and GITR agonist antibodies can reverse this immune tolerance, and show anti-tumor effect in multiple tumor models.
mRNA expression analysis:
Fig 1. RT-PCR analysis of the GITR gene. The mGITR mRNA was detected in both wild type C57BL/6 and heterozygous B-hGITR mice, while the hGITR mRNA was detected only in the heterozygous B-hGITR mice.
Protein expression analysis:
Fig 2. Splenocytes from both wild type (WT) C57BL/6 and heterozygous B-hGITR mice were analyzed by flow cytometry. Mouse GITR+ T cells were detected in both WT C57BL/6 and heterozygous B-hGITR mice, while human GITR+ T cells were only detected in the heterozygous B-hGITR mice.