|Strain Name||C57BL/6-Sirpatm1(hSIRPA)Cd47tm1(hCD47)/Bcgen||Common Name||B-hSIRPA/hCD47 mice|
|Catalog number (Males)||BDM010M||Catalog number (Females)||BDM010F|
CD47 (CD47 molecule) ;
SIRPA (Signal regulatory protein alpha
Protein expression analysis
Fig 1. Splenocytes from both wild t ype (WT) C57BL/6 and homozygous B-hSIRPA/hCD47 mice were analyzed by flow cytometry.
Mouse CD47+ cells were detected in the WT C57BL/6 mice, while human CD47 + cells were detected in the homozygous B-hSIRPA/hCD47 mice.
Fig 3. Splenocytes from both wild t ype (WT) C57BL/6 and homozygous B-hSIRPA/hCD47 mice were analyzed by flow cytometry.
Mouse SIRPα+ cells were detected in non-T and non-B cells from both WT C57BL/6 and the homozygous B-hSIRPA/hCD47 mice, while human SIRPα+ cells were only detected in non-T and non-B cells from the homozygous B-hSIRPA/hCD47 mice. This might result from the cross recognition of hSIRPA by anti-mSIRPA antibodies.