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B-hSIRPA/hCD47 mice

2018-02-05 16:57:42.0

Strain description


Strain Name C57BL/6-Sirpatm1(hSIRPA)Cd47tm1(hCD47)/Bcgen  Common NameB-hSIRPA/hCD47 mice
Background  C57BL/6Catalog number B-DM-010
Catalog number (Males) BDM010M Catalog number (Females) BDM010F

Related Genes


CD47 (CD47 molecule) ;

SIRPA (Signal regulatory protein alpha


Targeting strategy

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Protein expression analysis

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Fig 1. Splenocytes from both wild t ype (WT) C57BL/6 and homozygous B-hSIRPA/hCD47 mice were analyzed by flow cytometry.

Mouse CD47+ cells were detected in the WT C57BL/6 mice, while human CD47 + cells were detected in the homozygous B-hSIRPA/hCD47 mice.






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Fig 3. Splenocytes from both wild t ype (WT) C57BL/6 and homozygous B-hSIRPA/hCD47 mice were analyzed by flow cytometry.

Mouse SIRPα+ cells were detected in non-T and non-B cells from both WT C57BL/6 and the homozygous B-hSIRPA/hCD47 mice, while human SIRPα+ cells were only detected in non-T and non-B cells from the homozygous B-hSIRPA/hCD47 mice. This might result from the cross recognition of hSIRPA by anti-mSIRPA antibodies.


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